ThaumatinV1, Main, Exploration, bibRecord, 000485

Protein crystallisation on chemically modified mica surfaces.

Identifieur interne : 000485 ( Main/Exploration ); précédent : 000484; suivant : 000486

Protein crystallisation on chemically modified mica surfaces.

Auteurs : Giuseppe Falini [Italie] ; Simona Fermani ; Giovanna Conforti ; Alberto Ripamonti

Source :

Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2002.

RBID : pubmed:12351879

Descripteurs français

KwdFr :
- Animaux (MeSH), Concanavaline A (composition chimique), Cristallisation (méthodes), Lysozyme (composition chimique), Propriétés de surface (MeSH), Propylamines (MeSH), Protéines (composition chimique), Protéines végétales (composition chimique), Silanes (MeSH), Silicates d'aluminium (composition chimique), Taille de particule (MeSH), Édulcorants (composition chimique).
MESH :
- composition chimique : Concanavaline A, Lysozyme, Protéines, Protéines végétales, Silicates d'aluminium, Édulcorants.
- méthodes : Cristallisation.
- Animaux, Propriétés de surface, Propylamines, Silanes, Taille de particule.

English descriptors

KwdEn :
- Aluminum Silicates (chemistry), Animals (MeSH), Concanavalin A (chemistry), Crystallization (methods), Muramidase (chemistry), Particle Size (MeSH), Plant Proteins (chemistry), Propylamines (MeSH), Proteins (chemistry), Silanes (MeSH), Surface Properties (MeSH), Sweetening Agents (chemistry).
MESH :
- chemical , chemistry : Aluminum Silicates, Concanavalin A, Muramidase, Plant Proteins, Proteins, Sweetening Agents.
- methods : Crystallization.
- Animals, Particle Size, Propylamines, Silanes, Surface Properties.

Abstract

Chemically modified mica sheets have been tested as heterogeneous nucleant surfaces for lysozyme, concanavalin A and thaumatin. Smooth mica surfaces with reduced hydrophilic properties and different density of ionisable groups have been prepared by a silanisation reaction using mixtures of n-propyltriethoxysilane and 3-aminopropyltriethoxysilane in different percentages starting from 0 to 100% of aminosilane. The crystallisation experiments were carried out with the hanging drop vapour diffusion technique. The results suggest that these mica surfaces act as heterogeneous nucleant agents, whose effectiveness is due to non-specific attractive and local interactions between charged residues of the protein and the ionisable groups on the mica surfaces.

DOI: 10.1107/s0907444902012763
PubMed: 12351879

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Falini, Giuseppe" sort="Falini, Giuseppe" uniqKey="Falini G" first="Giuseppe" last="Falini">Giuseppe Falini</name>
<affiliation wicri:level="4"><nlm:affiliation>Dipartimento di Chimica "G Ciamician" Alma Mater Studiorum Università di Bologna, via Selmi 2, I-40126 Bologna, Italy. falini@ciam.unibo.it</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Dipartimento di Chimica "G Ciamician" Alma Mater Studiorum Università di Bologna, via Selmi 2, I-40126 Bologna</wicri:regionArea>
<orgName type="university">Université de Bologne</orgName>
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<author><name sortKey="Fermani, Simona" sort="Fermani, Simona" uniqKey="Fermani S" first="Simona" last="Fermani">Simona Fermani</name>
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<author><name sortKey="Conforti, Giovanna" sort="Conforti, Giovanna" uniqKey="Conforti G" first="Giovanna" last="Conforti">Giovanna Conforti</name>
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<author><name sortKey="Ripamonti, Alberto" sort="Ripamonti, Alberto" uniqKey="Ripamonti A" first="Alberto" last="Ripamonti">Alberto Ripamonti</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum Silicates (chemistry)</term>
<term>Animals (MeSH)</term>
<term>Concanavalin A (chemistry)</term>
<term>Crystallization (methods)</term>
<term>Muramidase (chemistry)</term>
<term>Particle Size (MeSH)</term>
<term>Plant Proteins (chemistry)</term>
<term>Propylamines (MeSH)</term>
<term>Proteins (chemistry)</term>
<term>Silanes (MeSH)</term>
<term>Surface Properties (MeSH)</term>
<term>Sweetening Agents (chemistry)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Concanavaline A (composition chimique)</term>
<term>Cristallisation (méthodes)</term>
<term>Lysozyme (composition chimique)</term>
<term>Propriétés de surface (MeSH)</term>
<term>Propylamines (MeSH)</term>
<term>Protéines (composition chimique)</term>
<term>Protéines végétales (composition chimique)</term>
<term>Silanes (MeSH)</term>
<term>Silicates d'aluminium (composition chimique)</term>
<term>Taille de particule (MeSH)</term>
<term>Édulcorants (composition chimique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Aluminum Silicates</term>
<term>Concanavalin A</term>
<term>Muramidase</term>
<term>Plant Proteins</term>
<term>Proteins</term>
<term>Sweetening Agents</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Concanavaline A</term>
<term>Lysozyme</term>
<term>Protéines</term>
<term>Protéines végétales</term>
<term>Silicates d'aluminium</term>
<term>Édulcorants</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Crystallization</term>
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<term>Particle Size</term>
<term>Propylamines</term>
<term>Silanes</term>
<term>Surface Properties</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Propriétés de surface</term>
<term>Propylamines</term>
<term>Silanes</term>
<term>Taille de particule</term>
</keywords>
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<front><div type="abstract" xml:lang="en">Chemically modified mica sheets have been tested as heterogeneous nucleant surfaces for lysozyme, concanavalin A and thaumatin. Smooth mica surfaces with reduced hydrophilic properties and different density of ionisable groups have been prepared by a silanisation reaction using mixtures of n-propyltriethoxysilane and 3-aminopropyltriethoxysilane in different percentages starting from 0 to 100% of aminosilane. The crystallisation experiments were carried out with the hanging drop vapour diffusion technique. The results suggest that these mica surfaces act as heterogeneous nucleant agents, whose effectiveness is due to non-specific attractive and local interactions between charged residues of the protein and the ionisable groups on the mica surfaces.</div>
</front>
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<Title>Acta crystallographica. Section D, Biological crystallography</Title>
<ISOAbbreviation>Acta Crystallogr D Biol Crystallogr</ISOAbbreviation>
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<ArticleTitle>Protein crystallisation on chemically modified mica surfaces.</ArticleTitle>
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<Abstract><AbstractText>Chemically modified mica sheets have been tested as heterogeneous nucleant surfaces for lysozyme, concanavalin A and thaumatin. Smooth mica surfaces with reduced hydrophilic properties and different density of ionisable groups have been prepared by a silanisation reaction using mixtures of n-propyltriethoxysilane and 3-aminopropyltriethoxysilane in different percentages starting from 0 to 100% of aminosilane. The crystallisation experiments were carried out with the hanging drop vapour diffusion technique. The results suggest that these mica surfaces act as heterogeneous nucleant agents, whose effectiveness is due to non-specific attractive and local interactions between charged residues of the protein and the ionisable groups on the mica surfaces.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Falini</LastName>
<ForeName>Giuseppe</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Dipartimento di Chimica "G Ciamician" Alma Mater Studiorum Università di Bologna, via Selmi 2, I-40126 Bologna, Italy. falini@ciam.unibo.it</Affiliation>
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<Author ValidYN="Y"><LastName>Fermani</LastName>
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<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
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<Month>09</Month>
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<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Acta Crystallogr D Biol Crystallogr</MedlineTA>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000538">Aluminum Silicates</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
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<Chemical><RegistryNumber>0</RegistryNumber>
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<NameOfSubstance UI="D003208">Concanavalin A</NameOfSubstance>
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<Chemical><RegistryNumber>53850-34-3</RegistryNumber>
<NameOfSubstance UI="C003427">thaumatin protein, plant</NameOfSubstance>
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<Chemical><RegistryNumber>EC 3.2.1.17</RegistryNumber>
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<Chemical><RegistryNumber>L8S6UBW552</RegistryNumber>
<NameOfSubstance UI="C477625">amino-propyl-triethoxysilane</NameOfSubstance>
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Protein crystallisation on chemically modified mica surfaces.

Protein crystallisation on chemically modified mica surfaces.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki